HydroLIT is a manual that proposes innovative strategies for improved water quality in the southeast Tennessee region. These strategies acknowledge the ability of watershed boundaries to offer a framework for merging visions of urban development and ecological health. They coalesce the roles of economic prosperity and natural resource health in shaping urban life as well as the relationship between designed interventions and their surrounding biophysical flows. These proposals function at multiple scales and offer tactical interventions as economical first steps for local communities to begin implementation. HydroLIT is a pioneering toolkit that aims to empower citizens and policymakers with knowledge of water quality stewardship that can affect change across the region.